Delta inulin: a novel, immunologically active, stable packing structure comprising β-d-[2 → 1] poly(fructo-furanosyl) α-d-glucose polymers

摘要: We report a novel isoform of β-D-[2 → 1] poly(fructo-furanosyl) α-D-glucose termed delta inulin (DI), comparing it with previously described alpha (AI), beta (BI) and gamma (GI) isoforms. In vitro, DI is the most immunologically active weight/weight in human complement activation binding to monocytes regulating their chemokine production cell surface protein expression. vivo, this translates into potent immune adjuvant activity, enhancing humoral cellular responses against co-administered antigens. As biocompatible polysaccharide particle, safe well tolerated by subcutaneous or intramuscular injection. Physico-chemically, forms as an insoluble precipitate from aqueous solution suitable AI, BI GI held at 37-48°C, whereas same lower temperatures has properties AI GI. can also be produced heat conversion suspensions 56°C, converted 45°C. distinguished its higher temperature dilute suspension solubility dimethyl sulfoxide, both consistent greater hydrogen bonding DI's polymer packing structure. dissolved heat, re-precipitated cooling finally re-converted back repeated treatment. Thus, DI, like isoforms, reflects formation distinct aggregate structure via reversible noncovalent bonding. basis for new vaccine further swells growing family carbohydrate structures immunological activity.