Excited States of the 5-Chlorophyll Photosystem II Reaction Center

摘要: Results of 4.2 K hole burning, chemical reduction (sodium dithionite, in dark and with illumination), oxidation (ferricyanide) experiments are reported for the isolated PS II reaction center containing five chlorophyll (Chl) molecules (RC-5). Qy states at 679.6 668.3 nm identified as being highly localized on pheophytin a D1 branch (Pheo1) D2 (Pheo2), respectively. The Pheo1−Qx Pheo2−Qx transitions were found to lie low high energy sides single Pheo−Qx absorption band, 544.4 541.2 nm, band 684 absorbing Chl, which is more apparent RC-5 than RC-6 samples, assigned peripheral Chl side. results consistent that Chlz (Stewart, D. H.; Cua, A.; Chisholm, Diner, B. Bocian, F.; Brudvig, G. W. Biochemistry 1998, 37, 10040). indicate P680, primary electron donor, main acceptor transf...