Rapid diagnosis of influenza type A infection : Comparison of shell-vial culture, directigen FLU-A and enzyme-linked immunosorbent assay

摘要: Two direct antigen detection methods (Directigen FLU A, Becton-Dickinson, Cokeysville, Md. and Influenza A virus ELISA Antigen kit, Alpha Biotech, Milano) were compared with isolation by shell-vial cultures for rapid diagnosis of influenza type infection. The three performed using a reference viral suspension consisting clinical isolate as well 41 nasopharyngeal aspirates collected from patients symptoms. was recovered 14 specimens shell-vials. Ten positive Directigen 7 kit. When on samples the sensitivity 64.2% 42.8%, respectively. limit shell-vials, stock titrated haemagglutinin activity, 0.06-0.03 HA units/ml at 24 hours or 72 after inoculation. detected containing 0.25 1 units/ml,