Phylogenetic utility of 141 low-copy nuclear regions in taxa at different taxonomic levels in two distantly related families of rosids.

摘要: Angiosperm systematics has progressed to the point where it is now expected that multiple, independent markers be used in phylogenetic studies. Universal primers for amplifying informative regions of chloroplast genome are readily available, but faster-evolving nuclear challenging discover priming sites conserved across distantly related taxa. With goals including identification rosids, and perhaps other angiosperms, we screened 141 primer combinations utility two distinct groups rosids at different taxonomic levels-Psiguria (Cucurbitaceae) Geraniaceae. We discovered three phylogenetically Psiguria Geraniaceae, none were useful both groups. Extending beyond combined our findings with those another recent effort testing these pairs Asteraceae, Brassicaceae, Orchidaceae. From this comparison, identified 32 amplified representative species least five angiosperm families, giving some prior indication about usefulness flowering plants. This reduced set low-copy along a recommended experimental strategy provide framework identifying angiosperms.