Tumor cell and immune cell profiles in primary human glioblastoma: Impact on patient outcome.

摘要: The distribution and role of tumor-infiltrating leucocytes in glioblastoma (GBM) remain largely unknown. Here, we investigated the cellular composition 55 primary (adult) GBM samples by flow cytometry correlated tumor immune profile with patient features at diagnosis outcome. single-cell suspensions were stained (n = 44) recurrence following radiotherapy chemotherapy (n = 11) a panel 8-color monoclonal antibody combinations for identification enumeration (GFAP+ CD45- ) normal astrocytic cells, infiltrating myeloid cells -i.e. microglial blood-derived tumor-associated macrophages (TAM), M1-like, M2-like TAM, neutrophils. myeloid-derived suppressor (MDSC)- lymphocytes (TIL) CD3+ T-cells their TCD4+ , TCD8+ TCD4- CD8- (CD25+ CD127lo regulatory (T-regs) subsets, (CD19+ CD20+ B-cells, (CD16+ NK-cells-. Overall, consisted major population (mean ± 1SD) (73% ± 16%) together significant but variable fraction (24% ± 18%). Within TAM predominated (13% ± 12%) including both (10% ± 11%) (3% ± 5%), addition to smaller proportion neutrophils (5% ± 9%) MDSC (4% ± 8%). Lymphocytes less represented mostly included (0.5% ± 0.7%) (0.6% ± 0.7%), lower numbers (0.2% ± 0.4%), T-regs (0.1% ± 0.2%), B-lymphocytes (0.1% ± 0.2%) NK-cells (0.05% ± 0.05%). three distinct profiles identified: cases minor leucocytes, tumors predominance neutrophils, mixed infiltration T-lymphocytes. Untreated patients lymphoid infiltrates showed significantly shorter overall survival versus other two groups, absence gains EGFR gene (p = 0.02). Here show that cell are systematically present GBM, highly levels profiles. Patients T-lymphoid worse