Gene expression levels assessed by CA1 pyramidal neuron and regional hippocampal dissections in Alzheimer's disease.

摘要: To evaluate molecular signatures of an individual cell type in comparison to the associated region relevant towards understanding pathogenesis Alzheimer's disease (AD), CA1 pyramidal neurons and surrounding hippocampal formation were microaspirated via laser capture microdissection (LCM) from neuropathologically confirmed AD age-matched control (CTR) subjects as well wild mouse brain using single population RNA amplification methodology coupled with custom-designed microarray analysis real-time quantitative polymerase-chain reaction (qPCR) validation. predominantly displayed downregulation classes transcripts related synaptic transmission versus CTR. Regional dissections several overlapping genes found neuronal expression, upregulation select indicative admixed types including glial-associated markers immediate-early death genes. Gene level distributions observed regional mice paralleled expression mosaics seen postmortem human tissue. Microarray was validated qPCR studies tissue sector obtained a model AD/Down syndrome (Ts65Dn mice) normal disomic (2N) littermates. Classes that have greater percentage overall hybridization signal intensity within tended be communication. The converse also found, such under represented neuron profiles relative dissections. These observations highlight dilution effect is likely occur conventional studies. Thus, specific intrinsic circuits will yield informative gene profile data may subthreshold and/or underrepresented admixture types.