Glutaraldehyde fixation chemistry: oxygen-consuming reactions.

摘要: Abstract In tissue fixed with glutaraldehyde, dissolved O2 is rapidly consumed by two processes: residual respiration and glutaraldehyde-induced chemical uptake. The nature of the chemistry which consumes during fixation was investigated studying model reactions glutaraldehyde amines homogenized suspensions. addition to solutions most primary ammonia stimulated rapid consumption. reaction (e.g., 25 mM ethanolamine, glycine, or methylamine) 50% in 15 20 s at 37 degrees C. initial rate uptake followed second-order kinetics respect amine concentration. total amount sufficient account for stoichiometric conversion pyridines. These data are consistent synthesis pyridine derivatives from glutaraldehyde-amine precursors last step an irreversible oxidation dihydropyridines muscle suspensions, chemically inhibited, significantly increased Thus, depleted both demands cross-linking process. Since these experiments were done under conditions commonly used fixation, hypoxia should be assumed exist biological preparations glutaraldehyde.