Identification of the Bursicon-Containing Neurones in Abdominal Ganglia of the Tobacco Hornworm, Manduca Sexta

摘要: The abdominal ganglion neurones responsible for the secretion of peptide hormone bursicon from transverse nerves tobacco hornworm Manduca sexta have been localized. Each unfused produced during adult development. was then transported to next posterior nerve subsequent release following emergence. Of two efferent pathways that connect ganglia nerves, only one had ultrastructural features consistent with a neurosecretory function. All axons in this pathway contained numerous, elementary, dense-cored granules diameters ranging 100 300 nm. In pharate adults, cobalt back-filling revealed groups cell bodies ganglia: three dorso-laterally and eight at mid-line. Hormonal activity present lateral regions but not mid-line regions. dorso-lateral region, four blueish could be seen living tissue. combined clusters cells both sides more than 60% entire ganglion. More careful dissection cluster detected presence pairs individual members. Individual dye-fills these blue demonstrated were back-filled nerve. fourth may identical neurone whose axon travels descending, contralateral connective exits nervous system via On basis dendritic geometry, each identified as unique, neurone.