Enhancing lipid production from crude glycerol by newly isolated oleaginous yeasts: strain selection, process optimization, and fed-batch strategy.

摘要: High lipid-accumulating yeast Trichosporonoides spathulata was newly isolated using crude glycerol as a sole carbon source. After process optimization in 5-L bioreactor equipped with pH control and aeration system, T. produced biomass of 11.3 g/L lipid 5.01 g/L content 44.3 % 10 % (w/v) supplemented only 0.5 % ammonium sulfate. A one-stage fed-batch feeding sulfate enhanced production up to 17.3 7.25 g/L, respectively, 41.9 %, while two-stage the second stage led lower 13.8 g/L but higher 7.78 g/L 56.4 %. The fatty acid composition that is similar plant oil indicates high potential use biodiesel feedstocks.