Uptake of AMP, ADP, and ATP in Escherichia coli W
作者： Kimiko WATANABE , Satsuki TOMIOKA , Kiyoko TANIMURA , Hisae OKU , Koichiro ISOI
DOI: 10.1271/BBB.100063
关键词:
摘要: The uptake activity ratio for AMP, ADP, and ATP in mutant (T-1) cells of Escherichia coli W, deficient de novo purine biosynthesis at a point between IMP 5-aminoimidazole-4-carboxiamide-1-β-D-ribofuranoside (AICAR), was 1:0.43:0.19. This approximately equal to the 5'-nucleotidase E. W cells. order inhibitory effect on [2-³H]ADP by T-1 adenine > adenosine AMP ATP. About 2-fold more radioactive bases than nucleosides were detected cytoplasm after 5 min an experiment with [8-¹⁴C]AMP Uptake [2-³H]adenosine inhibited inosine, but not (Ad-3) which lacked deaminase adenylosuccinate lyase. These experiments suggest that are converted mainly hypoxanthine via inosine before into
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