Nucleotide sequence of the fusion and haemagglutinin-neuraminidase glycoprotein genes of Newcastle disease virus, strain Ulster: molecular basis for variations in pathogenicity between strains.

摘要: Summary The nucleotide sequences of the fusion (F) and haemagglutinin-neuraminidase (HN) glycoprotein genes extremely avirulent Newcastle disease virus (NDV) strain Ulster have been determined by sequencing cDNA clones derived from viral genomic RNA. Open reading frames, assumed to encode F0 HN0 precursors, were 553 616 amino acids long, respectively. Comparisons two with those more virulent NDV strains suggested an explanation for molecular basis wide-ranging differences in virulence observed between NDV. The open frame corresponding HN extended beyond C terminus strains. This C-terminal extension was be responsible origin precursor (HN0) found other There fewer basic at cleavage site than are present strains, which may absence activation this many host cells. In NDV, as well paramyxoviruses, a phenylalanine residue occurs N F1 fragment. occurrence leucine position partly lack strain.