Iodination-deiodination. A radiochemical method for detection of structure and changes in structure in RNA.

摘要: Abstract Bound iodine is released from radioiodinated nucleotides in polymers exposed to sodium bisulfite. The rate of bisulfite-catalyzed deiodination pyrimidines can be controlled both by change temperature or pH and also dependent on the molecular association nucleotide. release iodocytidine polycytidylate greater than elimination RNA. Experiments testing influence base-pairing iodopyrimidines synthetic polynucleotides showed that pairing substituted nucleotide protected bond. rates several RNAs were measured. action bisulfite all single stranded tested was multiphasic consisting a rapid early reaction supplanted slower phase which followed reacceleration release. double RNA DNA-RNA duplexes retarded comparison with ribosomal messenger fractions. profiles suggested intermediate stage governed melting paired zones low stability. Late may result destabilization molecule through addition pyrimidine ring deamination. effect substances expected complex tested. Acridine orange ethidium bromide increased loss but slightly decreased viral A basic protein fraction isolated particles accelerated While caused this an equal amount albumin, as non-specific. results show sensitivity chemical follow interaction small amounts polynucleotides.