Transgenic expression of trypsin inhibitor CMe from barley in indica and japonica rice, confers resistance to the rice weevil Sitophilus oryzae.

摘要: Indica and japonica rice (Oryza sativa L.) plants were transformed by particle bombardment with the Itr1 gene encoding barley trypsin inhibitor BTI-CMe, under control of its own promoter that confers endosperm specificity, maize ubiquitin promoter. From 38 independent transgenic lines indica (breeding line IR58) 15 (cv Senia) selected, 22 11, respectively, expressed at detectable levels. The transgene was correctly translated as indicated western blot analysis a level expression in R3 seeds up to 0.31% (IR58) 0.43% (Senia) total extracted protein. functional integrity BTI-CMe confirmed activity assays liquid media staining gels, performed seed extracts. significant reduction survival rate weevil (Sitophilus oryzae, Coleoptera: Curculionidae) reared on homozygous expressing compared non-transformed controls, decrease trypsin-like insect crude midgut extracts, utility this proteinase for important storage pests.