Time course of changes in in vitro lipolysis of intra-abdominal fat depots in relation to high-fat diet-induced hepatic steatosis in rats.
作者： Pascal Collin , Natalie Chapados , Elise Dufresne , Pierre Corriveau , Pascal Imbeault
DOI: 10.1079/BJN20061775
关键词:
摘要: The purpose of the present study was to determine time course changes in vitro lipolysis and perilipin content (Western blot) mesenteric and/or retroperitoneal fat depots relation development hepatic steatosis high-fat diet-fed rats. Female Sprague-Dawley rats were submitted a diet (HF diet; 42 % as kJ) or standard (SD diet) for 1, 2, 3 8 weeks. Fat accretion tissues higher (P,0·01) HF than SD soon 1 week after beginning diet. Liver triacylglycerol concentrations significantly throughout experiment, highest values being reached at 2 Basal stimulated (10 24 10 27 M-isoproterienol) not changed during first weeks, regardless Lipolysis adipose tissue basal states was, however, weeks diets. There no significant (P.0·05) effects on tissue. In spite rapid accretion, results do provide any evidence (3 weeks) increase intra-abdominal upon undertaking an where liver lipid infiltration is most significant. Perilipin: Plasma NEFA: Leptin: Hepatic steatosis: Isoproterenol-induced
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