Specificity of antinuclear autoantibodies recognizing the dense fine speckled nuclear pattern: Preferential targeting of DFS70/LEDGFp75 over its interacting partner MeCP2

摘要: Abstract Human antinuclear autoantibodies (ANAs) targeting the dense fine speckled (DFS) nuclear protein DFS70, commonly known as lens epithelium derived growth factor p75 (LEDGFp75), present a clinical puzzle since their significance remains elusive. While frequencies are low in ANA-positive autoimmune rheumatic diseases, they relatively elevated laboratory referrals, diverse inflammatory conditions, and ‘apparently’ healthy individuals. We reported previously that DFS70/LEDGFp75 is an autoantigen prostate cancer closely interacts with another 70 kD DFS protein, methyl CpG binding 2 (MeCP2). This led us to investigate if anti-DFS sera exclusively target or also recognize MeCP2. Using several complementary autoantibody detection platforms cellular/molecular approaches we evaluated 65 human producing autoantibodies. Our results show these antibodies highly specific for do not Establishing specificity of has implications increasing our understanding biological utility.