作者: Robert P. Gunsalus , R.S. Wolfe
DOI: 10.1016/0006-291X(77)91570-4
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摘要: Abstract Addition of methyl-coenzyme M (CH3SCH2CH2SO3−) to undialized, anaerobic, cell-extracts Methanobacterium thermoautotrophicum under an atmosphere H2 and CO2 (80:20 v/v) stimulates 30-fold the rate reduction methane. For each mol CH3SCH2CH2SO3− added 12 methane is produced. This stimulation phenomenon requires magnesium ion, ATP, H2, CH3SCH2CH2SO3−. Neither reduced form cofactor, HSCH2CH2SO3−, nor oxidized, disulfide will replace methylated coenzyme.