Plasmodium berghei: Uptake and distribution of chloroquine in infected mouse erythrocytes

摘要: The capacity of mouse erythrocytes infected with Plasmodium berghei to accumulate chloroquine is developed maturation the parasites. This shown by direct comparison early and mature stages, which are separated density difference. After drug accumulation, cells were fractionated saponin lysis or nitrogen decompression study distribution. Effectiveness isolating intact parasites host components was checked SDS-polyacrylamide gel electrophoresis low leakage parasite-specific lactate dehydrogenase used as a marker enzyme. At external concentration (~10−7M), principally accumulated in However, at higher concentrations (~10−5and ~10−3M), proportion found cytosol fraction increased. A small but significant (<20%) associated cell membrane. pellet freed parasites, further freeze-thaw lysis, contains major concentrations. obtained from prolonged sonication bulk hemozoin pigment, carries only drug. indicates that parasite membrane may bind most As increased, supernatant increases, some probably bound soluble hemecontaining compounds. presence does not affect partition heme particulate forms.