Budgett's frog (Lepidobatrachus laevis): A new amphibian embryo for developmental biology.

摘要: Abstract The large size and rapid development of amphibian embryos has facilitated ground-breaking discoveries in developmental biology. Here, we describe the embryogenesis Budgett's frog (Lepidobatrachus laevis), an unusual species with eggs that are over twice diameter laboratory Xenopus, can tolerate higher temperatures to develop into a tadpole four times more rapidly. In addition detailing their early development, demonstrate that, like these amenable explant culture assays express exogenous transcripts tissue-specific manner. Moreover, steep trajectory scale Lepidobatrachus make it exceptionally well-suited for morphogenesis research. For example, developing organs massive compared those most model species, composed larger individual cells, thereby affording increased subcellular resolution vertebrate organogenesis. Furthermore, found complete limb regeneration, which typically requires months achieve models, occurs matter days tadpole, substantially accelerates pace experimentation. Thus, combination greater speed provides inimitable advantages studies—and novel inroad address mechanisms spatiotemporal scaling during evolution.