Immunohistochemical Analysis of in Vivo Patterns of Bak Expression, a Proapoptotic Member of the Bcl-2 Protein Family

摘要: The in vivo patterns of bak gene expression were determined human tissues using an immunohistochemical approach. Polyclonal antisera raised rabbits against a synthetic peptide corresponding to amino acids 14-36 the Bak protein, and shown be specific by immunoblot analysis various cell lines. immunoreactivity was detected wide variety types typically present within cytosol punctuate pattern suggestive association with intracellular organelles. Consistent proapoptotic role for gradients protein production observed complex epithelia nasopharynx, esophagus, colon, bladder, immunointensity being highest upper layers relatively low basal portions these epithelia. Similarly, myeloid series hematopoietic cells, strongest terminally differentiated granulocytes, only weak immunostaining occurring most progenitor cells bone marrow. Among other prominent were: (a) cardiomyocytes; (b) vascular visceral smooth muscle cells; (c) prostate glands; (d) myoepithelial mammary (e) distal convoluted tubules kidney; (f) epidermal keratinocytes; (g) enterocytes small intestine; (h) Sertoli Leidig testes; (i) theca interna ovary; (j) adrenal cortex (but not medulla). Nearly all neurons glial central nervous system did contain immunodetectable whereas sympathetic as well dorsal root ganglia their axons immunopositive. Most circulating peripheral blood lymphocytes negative immunostaining, strong found frequently nodes spleen. Overall, are unique compared members bcl-2 family, suggest that regulates death at stages differentiation through tissue-specific control its expression.