作者: Mustafa Mir , Michael R. Stadler , Stephan A. Ortiz , Melissa M. Harrison , Xavier Darzacq
DOI: 10.1101/377812
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摘要: The regulation of transcription requires the coordination numerous activities on DNA, yet it remains poorly understood how factors facilitate these multiple functions. Here we use lattice light-sheet microscopy to integrate single-molecule and high-speed 4D imaging in developing Drosophila embryos study nuclear organization interactions key patterning Zelda Bicoid. In contrast previous studies suggesting stable, cooperative binding, show that both interact with DNA surprisingly high off-rates. We find form dynamic subnuclear hubs, Bicoid binding is enriched within hubs. Remarkably, hubs are short lived only transiently sites active dependent transcription. Based our observations hypothesize that, beyond simply forming bridges between machinery, can organize other proteins into drive at their gene targets.