Dynamic multifactor hubs interact transiently with sites of active transcription in Drosophila embryos

作者: Mustafa Mir , Michael R. Stadler , Stephan A. Ortiz , Melissa M. Harrison , Xavier Darzacq

DOI: 10.1101/377812

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摘要: The regulation of transcription requires the coordination numerous activities on DNA, yet it remains poorly understood how factors facilitate these multiple functions. Here we use lattice light-sheet microscopy to integrate single-molecule and high-speed 4D imaging in developing Drosophila embryos study nuclear organization interactions key patterning Zelda Bicoid. In contrast previous studies suggesting stable, cooperative binding, show that both interact with DNA surprisingly high off-rates. We find form dynamic subnuclear hubs, Bicoid binding is enriched within hubs. Remarkably, hubs are short lived only transiently sites active dependent transcription. Based our observations hypothesize that, beyond simply forming bridges between machinery, can organize other proteins into drive at their gene targets.

参考文章(93)
Mustafa Mir, Armando Reimer, Jenna E. Haines, Xiao-Yong Li, Michael Stadler, Hernan Garcia, Michael B. Eisen, Xavier Darzacq, Dense Bicoid hubs accentuate binding along the morphogen gradient Genes & Development. ,vol. 31, pp. 1784- 1794 ,(2017) , 10.1101/GAD.305078.117
Danielle C. Hamm, Elizabeth D. Larson, Markus Nevil, Kelsey E. Marshall, Eliana R. Bondra, Melissa M. Harrison, A conserved maternal-specific repressive domain in Zelda revealed by Cas9-mediated mutagenesis in Drosophila melanogaster. PLOS Genetics. ,vol. 13, ,(2017) , 10.1371/JOURNAL.PGEN.1007120
Xiao-Yong Li, Melissa Harrison, Tommy Kaplan, Michael Eisen, Establishment of regions of genomic activity during the Drosophila maternal to zygotic transition bioRxiv. pp. 006312- ,(2014) , 10.1101/006312
Colleen E Hannon, Shelby A Blythe, Eric F Wieschaus, Concentration dependent chromatin states induced by the bicoid morphogen gradient eLife. ,vol. 6, ,(2017) , 10.7554/ELIFE.28275
Cyril Favard, Ovidiu Radulescu, Mounia Lagha, Melissa M Harrison, Jeremy Dufourt, Katharine N Schulz, Matthieu Dejean, Lucas Morales, Antonio Trullo, Jennifer Hunter, Carola Fernandez, Jorge Lazaro, Saida Nait-Amer, Temporal control of gene expression by the pioneer factor Zelda through transient interactions in hubs bioRxiv. pp. 282426- ,(2018) , 10.1101/282426
Davide Normanno, Lydia Boudarène, Claire Dugast-Darzacq, Jiji Chen, Christian Richter, Florence Proux, Olivier Bénichou, Raphaël Voituriez, Xavier Darzacq, Maxime Dahan, None, Probing the target search of DNA-binding proteins in mammalian cells using TetR as model searcher Nature Communications. ,vol. 6, pp. 7357- 7357 ,(2015) , 10.1038/NCOMMS8357
Katharine N. Schulz, Eliana R. Bondra, Arbel Moshe, Jacqueline E. Villalta, Jason D. Lieb, Tommy Kaplan, Daniel J. McKay, Melissa M. Harrison, Zelda is differentially required for chromatin accessibility, transcription factor binding, and gene expression in the early Drosophila embryo Genome Research. ,vol. 25, pp. 1715- 1726 ,(2015) , 10.1101/GR.192682.115
Kathleen A. Burke, Abigail M. Janke, Christy L. Rhine, Nicolas L. Fawzi, Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II Molecular Cell. ,vol. 60, pp. 231- 241 ,(2015) , 10.1016/J.MOLCEL.2015.09.006