SGK regulates meiotic resumption in ovarian starfish oocytes via intracellular pH-increase and cyclin B-Cdk1 activation
作者： Enako Hosoda , Daisaku Hiraoka , Noritaka Hirohashi , Saki Omi , Takeo Kishimoto
DOI: 10.1101/499699
关键词:
摘要: Tight regulation of intracellular pH (pHi) is essential for biological processes. Fully-grown oocytes, having a large nucleus called the germinal vesicle, arrest at meiotic prophase-I. Upon hormonal stimulus, oocytes resume meiosis to acquire fertilizability. At this time, pHi increases through Na+/H+ exchanger activity. However, and function change remains obscure. Here we show that in starfish serum- glucocorticoid-regulated kinase (SGK) activated by PI3K/TORC2/PDK1 signaling after required increase cyclin B-Cdk1 activation. Furthermore, when clamped 6.7, corresponding unstimulated ovarian stimulus normally induced activation; thereafter, initiated vesicle breakdown (GVBD), but failed complete it. Thus, SGK-dependent likely prerequisite completion GVBD oocytes. We propose model SGK drives resumption concomitant cell-cycle machinery.
sci-hub.st 参考文章(67)
Shun-ichi Miyazaki, Setsuro Hirai, Fast polyspermy block and activation potential. Correlated changes during oocyte maturation of a starfish. Developmental Biology. ,vol. 70, pp. 327- 340 ,(1979) , 10.1016/0012-1606(79)90031-9
Kana Usui, Noritaka Hirohashi, Kazuyoshi Chiba, Involvement of mitogen‐activating protein kinase and intracellular pH in the duration of the metaphase I (MI) pause of starfish oocytes after spawning Development Growth & Differentiation. ,vol. 50, pp. 357- 364 ,(2008) , 10.1111/J.1440-169X.2008.01036.X
Edmund C. Jenkins, Shawon Debnath, Sophia Varriano, Stephen Gundry, Jimmie E. Fata, Na(+) /H(+) exchanger 1 (NHE1) function is necessary for maintaining mammary tissue architecture. Developmental Dynamics. ,vol. 243, pp. 229- 242 ,(2014) , 10.1002/DVDY.24032
Sandra Eltschinger, Robbie Loewith, TOR Complexes and the Maintenance of Cellular Homeostasis Trends in Cell Biology. ,vol. 26, pp. 148- 159 ,(2016) , 10.1016/J.TCB.2015.10.003
Chris Yun, Concerted Roles of SGK1 and the Na+/H+ Exchanger Regulatory Factor 2 (NHERF2) in Regulation of NHE3 Cellular Physiology and Biochemistry. ,vol. 13, pp. 29- 40 ,(2003) , 10.1159/000070247
Eiji Kinoshita, Emiko Kinoshita-Kikuta, Kei Takiyama, Tohru Koike, Phosphate-binding Tag, a New Tool to Visualize Phosphorylated Proteins Molecular & Cellular Proteomics. ,vol. 5, pp. 749- 757 ,(2006) , 10.1074/MCP.T500024-MCP200
Sherwin C. Lee, Richard A. Steinhardt, pH changes associated with meiotic maturation in oocytes of Xenopus laevis Developmental Biology. ,vol. 85, pp. 358- 369 ,(1981) , 10.1016/0012-1606(81)90267-0
Peijian He, Sei-Jung Lee, Songbai Lin, Ursula Seidler, Florian Lang, Geza Fejes-Toth, Aniko Naray-Fejes-Toth, C. Chris Yun, Serum- and glucocorticoid-induced kinase 3 in recycling endosomes mediates acute activation of Na+/H+ exchanger NHE3 by glucocorticoids Molecular Biology of the Cell. ,vol. 22, pp. 3812- 3825 ,(2011) , 10.1091/MBC.E11-04-0328
D Hiraoka, E Okumura, T Kishimoto, Turn motif phosphorylation negatively regulates activation loop phosphorylation in Akt Oncogene. ,vol. 30, pp. 4487- 4497 ,(2011) , 10.1038/ONC.2011.155
Juan M. García-Martínez, Dario R. Alessi, mTOR complex 2 (mTORC2) controls hydrophobic motif phosphorylation and activation of serum- and glucocorticoid-induced protein kinase 1 (SGK1) Biochemical Journal. ,vol. 416, pp. 375- 385 ,(2008) , 10.1042/BJ20081668