Discrimination and Quantification of Spectrally Similar Near-Infrared Probes by Time-Domain (TD) Optical Imaging in Acute Myeloid Leukemia Mouse Models.

摘要: The use of whole-body optical imaging in the near-infrared (NIR) spectrum (650–1100 nm) employing fluorescently labelled reagents recognising cell-specific biomarkers leukemia has become a standard modality preclinical models human disease. A particular challenge is represented by leukemic infiltrates liver and spleen, organs with high absorbance. While there are increasingly impressive arrays biomolecules available for exploitation via imaging, number commmercially availible fluorophores NIR remain limited. In particular, simultaneous disease progression functional more specific biological processes within same sample complicated requiste multiple filtersets similar spectral properties. Subsequent “bleeding” fluorescence through unavoidable precluding ones ability to quantify based on fluorescence. Similarly, descrimination vivo autofluorescence properties commonly employed dyes, consequent ingested food comlicates contrast even further. More recently techniques have aided discrimination profiles however, these attenuate much light reaching detector. Time-domain (TD) pulsed laser diodes time resolved detector system, typically photo-multiplier tube (PMT), previously been demonstrated distinguish between changes physiological such as; tissue pH or calcuim concentration, lifetime probe. Here we demonstrate single wave lenght TD (eXplore Optix™, ART Inc) potential discriminate combinations diverse probes spectrally but differing basis appropriate vitro phantoms. illustrate this technique endogenous from administered cells subsequent distinction mixtures their inherent fluorescent lifetimes vivo.