Microscopic visualization of cell – Cold sprayed bio-coating interfaces: An intermediate layer formed during the culturing mediates the behaviors of the cells

摘要: Abstract Cold spraying has been proved it is a promising method for biomaterials surface modification and additive manufacturing. Fundamental understanding of the cell-biomaterial interaction essential design fabrication their biomedical applications. Here we report evolutional characteristics interfaces between osteoblast cells cold sprayed hydroxyapatite-titanium composite coating. The in situ observation was carried out by focused ion beam-scanning electron microscopy, influence coatings on behaviors protein adsorption cell proliferation examined. Apparent cellular sensing, adhesion, migration, mineral dissolution after 3 h incubation were visualized. predominant existence fragment particles coatings, one exceptional features coating, plays vital role regulating association uptake. presence Ti HA fragments at cell-coating interface had no significant effect viability proliferation. After 5 days culturing, vitro fusion mineralization with formation an intermediate layer evidenced suggesting rapid regeneration accelerated establishment bone-implant integration. These critical phenomena occurred different periods culturing would facilitate specific responses, shed light development manufacturing techniques biomaterials.