Evidence for a role of osteopontin in macrophage infiltration in response to pathological stimuli in vivo

摘要: Previous studies have suggested that osteopontin, an arginine-glycine-aspartate-containing secreted protein, might be important in macrophage accumulation during interstitial nephritis and wound healing. The current study investigated the role of osteopontin intradermal infiltration using immunohistochemistry neutralizing antibodies. Purified induced after injection rat dermis facilitated adhesion migration cultured macrophage-like cells vitro. Intradermal N-formyl-met-leu-phe (FMLP), a potent chemotactic peptide, macrophage-rich infiltrate at site injection. Most these macrophages expressed high levels as shown by immunochemical analysis. To determine whether was required for their infiltration, we treated rats with either anti-osteopontin antibody (OP199) or nonimmune antibody. We found FMLP-induced largely inhibited (>60%) treatment compared receiving These data support hypothesis may critical mediator inflammation specific disease injury states, potentially promoting migration.