Changes in glycosylation of human bile-salt-stimulated lipase during lactation.
作者： Eva Landberg , Yunping Huang , Mats Strömqvist , Yehia Mechref , Lennart Hansson
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摘要: Abstract Bile-salt-stimulated lipase (BSSL) is an enzyme in human milk, which important for the fat digestion newborn infant. BSSL highly glycosylated and includes one site N-glycosylation several sites O-glycosylation. has previously been found to express Lewis a, b, x carbohydrate antigens. In this study, glycosylation of was studied at different times during lactation. purified from milk collected individually four donors first 6 months The glycans were characterized through monosaccharide analysis, high-pH anion-exchange chromatography, matrix-assisted laser desorption–ionization mass spectrometry, ELISA. Both total content relative amount sialic acid higher lactation month as compared later also showed a composition sialylated O-linked N-linked oligosaccharides consisted lower amounts fucosylated structures We gradual increase expression epitope on throughout period. This study shows that dependent blood group phenotype donor changes substantially
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