Vasoactive intestinal peptide-mediated suppression of apoptosis in the ovary: potential mechanisms of action and evidence of a conserved antiatretogenic role through evolution.

摘要: Vasoactive intestinal peptide (VIP)-containing nerve fibers are present in ovarian follicles at all stages of development, and VIP, acting primarily via the cAMP pathway, has been reported to modulate many aspects granulosa cell function. Herein we examined effects VIP its potential mechanisms action on apoptosis antral isolated from ovaries gonadotropin-primed immature rats incubated vitro under serum-free conditions. Additionally, avian cells were used as a comparative model system determine whether ability ovary conserved through evolution. Genomic DNA extracted rat exhibited extensive levels internucleosomal cleavage characteristic death apoptosis. Treatment with (1-1000 nM) caused dose-dependent reduction extent apoptotic breakdown, maximal effect a...