Functional Mapping of CD11b/CD18 Epitopes Important in Neutrophil-Epithelial Interactions: A Central Role of the I Domain

摘要: In the intestine, lung, and urinary tract, neutrophil (polymorphonuclear leukocyte, PMN) transepithelial migration is dependent on leukocyte beta2 integrin CD11b/CD18. While regions of CD11b involved in recognition several soluble ligands are known, those that mediate PMN-epithelial interactions have not been investigated. this study, mAbs reactive with four extracellular CD11b, NH2-terminal region, I (inserted) domain, cation-binding region proximal to transmembrane domain (C domain), were analyzed for ability block CD11b/CD18-mediated T84 intestinal epithelial cells. such a manner, epitope mapping was applied complex between CD11b/CD18 cell-based ligand system. Abs strongly inhibited both adhesion PMN cells across monolayers. However, profile inhibition distinct from other known CBRM1/32, an Ab discontinuous residing within NH2- domains, transmigration responses. C had minimal effects transmigration. These findings appear applicable epithelia, since similar results obtained experiments CF15 human airway Finally, profiles confirmed assays isolated purified demonstrate central role participation shared by domains mediating PMN-adhesive