Quantitative Thin-Layer Chromatography/Mass Spectrometry Analysis of Caffeine Using a Surface Sampling Probe Electrospray Ionization Tandem Mass Spectrometry System

摘要: Quantitative determination of caffeine on reversed-phase C8 thin-layer chromatography plates using a surface sampling electrospray ionization system with tandem mass spectrometry detection is reported. The chromatography/electrospray method employed deuterium-labeled internal standard and selected reaction monitoring detection. Up to nine parallel bands single plate were sampled in scanning experiment requiring 35 min at scan rate 44 μm/s. A HPLC/UV assay was developed assess the performance. Limits for methods determined from calibration curve statistics 0.20 ng injected (0.50 μL) 1.0 spotted plate, respectively. Spike recoveries standards real samples ranged between 97 106% both methods. content three die...