A simple assay for quantification of protein in tissue sections, cell cultures, and cell homogenates, and of protein immobilized on solid surfaces

摘要: The determination of total protein is often a key step for the quantitative analysis various parameters in tissue and general biochemical research. classical protocols are restricted to few compatible buffers, solutions containing agglomerates or immobilized on solid surfaces not available. In such cases, quantification may be complicated. Here, we describe simple sensitive method circumventing all these restrictions. Proteins solution suspension any buffer spotted onto cellulose acetate, dried, stained with Amido Black. After washing off excess dye, bound Black solubilized an acidic determined photometrically. Tissue slices (fixed native), adherent cell cultures, Western blots can also their content irrespective supporting material. A micro-version protocol proteins allows large numbers samples evaluated at time microtitration plates requires only 1–2 μl per sample. linear concentration dependency (r2=0.950–0.999) was obtained cases investigated. presented here permits exact soluble variety insoluble present wide supports, even whole cells slices.