Diversity of cyclic antimicrobial lipopeptides from Bacillus P34 revealed by functional annotation and comparative genome analysis

摘要: Cyclic lipopeptides (CLPs) from Bacillus strains have demonstrated a wide range of bioactivities making them interesting candidates for different applications in the pharmaceutical, food and biotechnological industries. Genome sequencing, together with phylogenetic analysis sp. P34, isolated freshwater fish gut, showed that bacterial strain belongs to velezensis group. In silico investigation metabolic gene clusters nonribosomal peptide synthetases (NRPS) revealed genetic elements associated synthesis surfactin, fengycin iturin family component bacillomycin. Further, an assay was conducted investigate production CLPs presence heat inactivated cultures or fungal spores. Maximum concentration observed at 24 h (2300-2700 mg/mL), while maximum amounts were detected 48 (250 mg/mL) heat-inactivated spores Aspergillus niger. Heat-inactivated cells Listeria monocytogenes caused reduction both amounts. The fengycins A B bacillomycin L confirmed by mass spectrometry analyses. This study reinforces potential B. P34 as valuable recognized important antimicrobial substances.