Regulation of Transcription by Ubiquitination without Proteolysis
作者： Peter Kaiser , Karin Flick , Curt Wittenberg , Steven I. Reed
DOI: 10.1016/S0092-8674(00)00036-2
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摘要: Abstract Polyubiquitination of proteins by Cdc34/SCF complexes targets them for degradation the 26S proteasome. The essential F-box protein Met30 is substrate recognition subunit ubiquitin ligase SCF . critical target transcription factor Met4, as deletion MET4 suppresses lethality met30 mutants. Surprisingly, Met4 a relatively stable and its abundance not influenced Met30. However, transcriptional repression genes correlates with Cdc34 /SCF -dependent ubiquitination Met4. Functionally, ubiquitinated associates promoters but fails to form functional complexes. Our data reveal novel proteolysis-independent function indicate that factors can be utilized directly regulate their activities.
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