Recent advances in antiviral research: identification of inhibitors of the herpesvirus proteases.

摘要: Major advances have been reported in the last two years regarding molecular biology and structural properties of herpesvirus proteases. X-ray diffraction studies enabled several groups to solve structure human cytomegalovirus protease. Fluorescence-based substrate assays also recently reported. These substrates exhibit sufficient kinetic sensitivity enable high-throughput screening efforts dedicated toward discovery protease inhibitors. Three classes inhibitors recently: nonpeptidic aryl trifluoromethylketones; alternate (benzoxazinones/azalactones); thiol-modifying The class offers a unique opportunity discover specific protease, as this requires reduced cysteine residues for its enzymatic activity.