作者: S⊘ren KRISTIANSEN , Toolsie RAMLAL , Amira KLIP
DOI: 10.1042/BJ3350351
关键词:
摘要: Insulin stimulates the rate of glucose transport into muscle and adipose cells by translocation transporter (GLUT4)-containing vesicles from an intracellular storage pool to surface membrane. This event is mediated through insulin receptor substrates (IRSs), which in turn activate phosphatidylinositol (PI) 3-kinase isoforms. It has been suggested that causes attachment PI 3-kinases GLUT4-containing rat cells. Furthermore, it also shown contain a 4-kinase. In present study we investigate whether isolated skeletal display and/or 4-kinase activities. stimulation caused rapid increase (5-15-fold compared with control) cytosolic IRS-1-associated PI-3 kinase activity. activity was membrane preparation containing insulin-regulatable GLUT4 transporters. However, when were immunoprecipitation basal insulin-stimulated (3 min) muscle, displayed 4-kinase, but not 3-kinase, did regulate vesicles. conclusion, 3-kinase. that, activation IRS/PI complex compartment associated closely vesicles, themselves.