Phosphatidylinositol 4-kinase, but not phosphatidylinositol 3-kinase, is present in GLUT4-containing vesicles isolated from rat skeletal muscle

作者: S⊘ren KRISTIANSEN , Toolsie RAMLAL , Amira KLIP

DOI: 10.1042/BJ3350351

关键词:

摘要: Insulin stimulates the rate of glucose transport into muscle and adipose cells by translocation transporter (GLUT4)-containing vesicles from an intracellular storage pool to surface membrane. This event is mediated through insulin receptor substrates (IRSs), which in turn activate phosphatidylinositol (PI) 3-kinase isoforms. It has been suggested that causes attachment PI 3-kinases GLUT4-containing rat cells. Furthermore, it also shown contain a 4-kinase. In present study we investigate whether isolated skeletal display and/or 4-kinase activities. stimulation caused rapid increase (5-15-fold compared with control) cytosolic IRS-1-associated PI-3 kinase activity. activity was membrane preparation containing insulin-regulatable GLUT4 transporters. However, when were immunoprecipitation basal insulin-stimulated (3 min) muscle, displayed 4-kinase, but not 3-kinase, did regulate vesicles. conclusion, 3-kinase. that, activation IRS/PI complex compartment associated closely vesicles, themselves.

参考文章(26)
A. R. Saltiel, Diverse signaling pathways in the cellular actions of insulin American Journal of Physiology-endocrinology and Metabolism. ,vol. 270, ,(1996) , 10.1152/AJPENDO.1996.270.3.E375
K.J. Rodnick, J.W. Slot, D.R. Studelska, D.E. Hanpeter, L.J. Robinson, H.J. Geuze, D.E. James, Immunocytochemical and biochemical studies of GLUT4 in rat skeletal muscle. Journal of Biological Chemistry. ,vol. 267, pp. 6278- 6285 ,(1992) , 10.1016/S0021-9258(18)42692-0
M J Quon, A J Butte, M J Zarnowski, G Sesti, S W Cushman, S I Taylor, Insulin receptor substrate 1 mediates the stimulatory effect of insulin on GLUT4 translocation in transfected rat adipose cells. Journal of Biological Chemistry. ,vol. 269, pp. 27920- 27924 ,(1994) , 10.1016/S0021-9258(18)46875-5
J W Biber, G E Lienhard, Isolation of vesicles containing insulin-responsive, intracellular glucose transporters from 3T3-L1 adipocytes Journal of Biological Chemistry. ,vol. 261, pp. 16180- 16184 ,(1986) , 10.1016/S0021-9258(18)66695-5
Jih-I Yeh, Eric A. Gulve, Lucia Rameh, Morris J. Birnbaum, The effects of wortmannin on rat skeletal muscle. Dissociation of signaling pathways for insulin- and contraction-activated hexose transport. Journal of Biological Chemistry. ,vol. 270, pp. 2107- 2111 ,(1995) , 10.1074/JBC.270.5.2107
R.L. Del Vecchio, P.F. Pilch, Phosphatidylinositol 4-kinase is a component of glucose transporter (GLUT 4)-containing vesicles. Journal of Biological Chemistry. ,vol. 266, pp. 13278- 13283 ,(1991) , 10.1016/S0021-9258(18)98835-6
Qinghua WANG, Philip J. BILAN, Theodoros TSAKIRIDIS, Aleksander HINEK, Amira KLIP, Actin filaments participate in the relocalization of phosphatidylinositol3-kinase to glucose transporter-containing compartments and in the stimulation of glucose uptake in 3T3-L1 adipocytes Biochemical Journal. ,vol. 331, pp. 917- 928 ,(1998) , 10.1042/BJ3310917
K V Kandror, L Coderre, A V Pushkin, P F Pilch, Comparison of glucose-transporter-containing vesicles from rat fat and muscle tissues: evidence for a unique endosomal compartment. Biochemical Journal. ,vol. 307, pp. 383- 390 ,(1995) , 10.1042/BJ3070383
Taro OKADA, Osamu HAZEKI, Michio UI, Toshiaki KATADA, Synergistic activation of PtdIns 3-kinase by tyrosine-phosphorylated peptide and beta gamma-subunits of GTP-binding proteins. Biochemical Journal. ,vol. 317, pp. 475- 480 ,(1996) , 10.1042/BJ3170475