Metagenomic signature of natural strongyle infection in susceptible and resistant horses

摘要: Gastrointestinal strongyles are a major threat to horses9 health and welfare. Given that inhabit the same niche as gut microbiota, they may interact with each other. These beneficial or detrimental interactions unknown in horses could partly explain contrasted susceptibility infection between individuals. To address these questions, an experimental pasture trial 20 worm-free female Welsh ponies (10 susceptible (S) 10 resistant (R) parasite infection) was implemented for five months. Fecal egg counts (FEC), hematological biochemical data, body weight microbiota composition were studied individual after 0, 24, 43, 92 132 grazing days. The predicted R exhibited lower FEC days, showed higher levels of circulating monocytes eosinophils, while S developed lymphocytosis by end trial. Although overall diversity remained similar two groups, sustained differential abundances Clostridium XIVa, Ruminococcus, Acetivibrio unclassified Lachnospiracea at day 0. bacteria hence contribute intrinsic pony resistance towards strongyle infection. Moreover, Paludibacter, Campylobacter, Bacillus, Pseudomonas, III, Acetivibrio, members Eubacteriaceae Ruminococcaceae fungi loads increased infected ponies, suggesting other success ecological equine intestines. In contrast, butyrate-producing such XIVa Lachnospiraceae family decreased relative ponies. Additionally, alterations induced changes several immunological pathways including pathogen sensing, lipid metabolism, activation signal transduction critical regulation immune system energy homeostasis. observations shed light on putative implication Overall, this longitudinal study provides foundation better understand mechanisms underpin relationship host infection, response under natural conditions should development novel biomarkers provide additional control options.