Detection of Functional VH81X Heavy Chains in Adult Mice with an Assessment of Complementarity-Determining Region 3 Diversity and Capacity to Form Pre-B Cell Receptor

摘要: Recent reports have indicated that up to 50% of all H chain proteins formed cannot associate with the surrogate L complex and therefore fail form a pre-B cell receptor (pBCR), which is required for allelic exclusion and, in most cases, verifies can assemble an Ab molecule. Certain VH genes, such as VH81X, appear be particularly prone encoding nonpairing (dysfunctional) chains. It has been suggested sequence variability at complementarity-determining region 3, especially when increased by enzyme TdT, often precludes ability VH81X-using chains pBCR. To determine whether motif exists accounts pair complex/L chain, we bred mouse line recombination only occur on one allele, allowing us compile pool capable forming molecules absence dysfunctional Somewhat unexpectedly, found VH81X formation surface expression presence TdT. Scrutiny these revealed that, although highly encode chains, not severely limited among functional We also demonstrate Ig indicative capacity