[21] Bacterial collagenase

摘要: Publisher Summary This chapter discusses purification, specificity and physical properties of the bacterial enzymes focuses on use enzyme for analysis identification collagen species. Purification sequence is discussed from standpoint obtaining an preparation free other proteolytic activity capable quantitatively specifically digesting in a mixture proteins. Limited digestion with collagenases can be used to obtain information helical region collagen. The site cleavage determined by electron microscopy segment long-spacing fragments or Edman degradation. At low enzyme-to-substrate ratio, occurred mainly at two sites—interbands 26–27 33–34 calf skin collagen, but bands 18 22 rat Highly purified A. iophagus collagenase produced cleavages 33-34 41 both types