WITHDRAWN: Demethylation of Di-Methylation of Lysine 4 on Histone 3 Is Inhibited by General Control Nondepressible 5-Induced Acetylation of Lysine-Specific Demethylase 1

摘要: ABSTRACT Background Histone demethylase lysine-specific 1 (LSD1) potentiates cancer progression through inducing demethylation of di-methylation lysine 4 on histone 3. Here, we aimed to investigate whether general control nondepressible 5 (GCN5) has an activity induce acetylation LSD1, and the function acetylated LSD1 was further explored. Materials Methods Immunoblotting applied detect GCN5 in colorectal adenocarcinoma cells (SW48 SNU-C1). or expression altered by transfection. Coimmunoprecipitation glutathione S-transferase pulldown assays were carried out examine association between GCN5. Activity acetylate determined vitro.The examined, as well alteration histone, immunoblotting assay. The complex explored assessing cellular growth tumor volume mice. Results SW48 SNU-C1 cells, which accompanied high induced at 433 site. not affected GCN5-induced acetylation. However, associate with nucleosome. promoted increased colon cancer, whereas maintained methylation repressed progression. Conclusions impeded inhibiting histone.