Examination of poultry litter for Salmonellae by direct culture and fluorescent antibody technique.

摘要: Direct culture and fluorescent antibody (FA) procedures were used for examining approximately 6200 samples of poultry floor or nest litter salmonellae. Each sample was cultured directly in tetrathionate brilliant-green (TBG) broth, incubated at 42 C 48 hours, plated on brilliant green (BG) agar. The FA procedure conducted from pools 4-8 prepared by transferring 1 ml supernatant TBG 24 hr into 50 either gram-negative (GN) selenite cystine, sulfapyridine. After 6 incubation C, BG agar plates streaked each pool, smears FA. Salmonella isolated plating 268 854 pooled units. Recovery 97% direct culture, 86% GN 57% the secondary enrichments. In procedure, broth superior to selenites as medium which prepare smears, giving more FA-positive pools, a higher percentage confirmed positives, lower false negative pools. examination can be expedited using A major deficiency technique high false-positive reactions judged inability isolate From TBG, salmonellae total number culturally positive combined methods.