Escherichia coli YafP protein modulates DNA damaging property of the nitroaromatic compounds.

摘要: Escherichia coli SOS functions constitute a multifaceted response to DNA damage. We undertook study the role of yafP, gene with unknown function. yafP is part an operon also containing dinB coding for Polymerase IV (PolIV). Our phylogenetic analysis showed that content this variable but and genes are conserved in majority E. natural isolates. Therefore, we studied if these proteins functionally linked. Using murine septicaemia model, YafP activity reduced bacterial fitness absence PolIV. Similarly, increased cytotoxicity two damaging nitroaromatic compounds, 4-nitroquinoline-1-oxide (NQO) nitrofurazone, The fact PolIV counterbalances YafP-induced could explain why transcriptionally involvement genotoxic-stress induced mutagenesis found NQO-induced mutagenicity. antimutator was independent activity. Given annotated as putative acetyltransferase, it be participates metabolic transformation genotoxic hence modulating balance between their mutagenicity cytotoxicity.