Annotating genomes with massive-scale RNA sequencing.
作者： France Denoeud , Jean-Marc Aury , Corinne Da Silva , Benjamin Noel , Odile Rogier
DOI: 10.1186/GB-2008-9-12-R175
关键词:
摘要: Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty aligning short reads on exon-exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, that is, in absence a set known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at directly from and demonstrate its utility grapevine genome.
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