Localization of P42 and F1-ATPase α-Subunit Homolog of the Gliding Machinery in Mycoplasma mobile Revealed by Newly Developed Gene Manipulation and Fluorescent Protein Tagging
作者： I. Tulum , M. Yabe , A. Uenoyama , M. Miyata
DOI: 10.1128/JB.01418-13
关键词:
摘要: Mycoplasma mobile has a unique mechanism that enables it to glide on solid surfaces faster than any other gliding mycoplasma. To elucidate the mechanism, we developed transformation system for M. based transposon derived from Tn4001. Modification of electroporation conditions, outgrowth time, and colony formation standard method species enabled successful transformation. A fluorescent-protein tagging technique was using enhanced yellow fluorescent protein (EYFP) applied two proteins have been suggested be involved in mechanism: P42 (MMOB1050), which is transcribed as continuous mRNA with essential gliding, homolog F1-ATPase α-subunit (MMOB1660). Analysis amino acid sequence by PSI-BLAST evolved common ancestor FtsZ, bacterial tubulin homologue. The roles subunit are discussed part our proposed mechanism.
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