Abundance of poly (a) binding protein in bovine oocytes during in vitro maturation
作者： Tatjana Smiljakovic , M. Max , W. Tomek
DOI: 10.2298/BAH0404005S
关键词:
摘要: To improve the conditions for in vitro production of embryos animal husbandry it can be helpful to obtain deeper insights into regulatory mechanisms which are directing maturation oocytes on a molecular level. Recent observations have shown that protein synthesis mature (metaphase II stage) is impaired. Although possess stockpile mRNA at this stage development, translation these mRNAs repressed. It has been essential components cap dependent regulation (eIF4E, 4E-BP1) potentially could attend process. More recent investigation suggest factors probably not alone modulate rates during bovine oocytes. Therefore present study abundance and potential modifications another regulator translation, namely poly (A)-binding was analysed. This bind poly-(A)tails link them cap-binding complex eIF4F. By mechanism, should by synergetically stimulated. In study, we analysed (A) binding course meiotic elucidated expression isoforms or post translational 2D-Gelelectrophoresis.
sci-hub.st 参考文章(16)
Gordon M. Cragg, David J. Newman, Stringner Sue Yang, Bioprospecting for drugs Nature. ,vol. 393, pp. 301- 301 ,(1998) , 10.1038/30586
Motoaki Wakiyama, Hiroaki Imataka, Nahum Sonenberg, Interaction of eIF4G with poly(A)-binding protein stimulates translation and is critical for Xenopus oocyte maturation Current Biology. ,vol. 10, pp. 1147- 1150 ,(2000) , 10.1016/S0960-9822(00)00701-6
S. Z. Tarun, S. E. Wells, J. A. Deardorff, A. B. Sachs, Translation initiation factor eIF4G mediates in vitro poly(A) tail-dependent translation Proceedings of the National Academy of Sciences of the United States of America. ,vol. 94, pp. 9046- 9051 ,(1997) , 10.1073/PNAS.94.17.9046
W Tomek, H Torner, W Kanitz, Comparative analysis of protein synthesis, transcription and cytoplasmic polyadenylation of mRNA during maturation of bovine oocytes in vitro. Reproduction in Domestic Animals. ,vol. 37, pp. 86- 91 ,(2002) , 10.1046/J.1439-0531.2002.00336.X
Hanh Le, Karen S. Browning, Daniel R. Gallie, The Phosphorylation State of Poly(A)-binding Protein Specifies Its Binding to Poly(A) RNA and Its Interaction with Eukaryotic Initiation Factor (eIF) 4F, eIFiso4F, and eIF4B Journal of Biological Chemistry. ,vol. 275, pp. 17452- 17462 ,(2000) , 10.1074/JBC.M001186200
Angelika Görg, Günther Boguth, Christian Obermaier, Walter Weiss, Two‐dimensional electrophoresis of proteins in an immobilized pH 4–12 gradient Electrophoresis. ,vol. 19, pp. 1516- 1519 ,(1998) , 10.1002/ELPS.1150190850
K. C. Kleene, M-Y. Wang, M. Cutler, C. Hall, D. Shih, Developmental expression of poly(A) binding protein mRNAs during spermatogenesis in the mouse. Molecular Reproduction and Development. ,vol. 39, pp. 355- 364 ,(1994) , 10.1002/MRD.1080390403
H. Torner, M. Kubelka, B. Heleil, W. Tomek, H. Alm, T. Kuzmina, V. Guiard, Dynamics of meiosis and protein kinase activities in bovine oocytes correlated to prolactin treatment and follicle size. Theriogenology. ,vol. 55, pp. 885- 899 ,(2001) , 10.1016/S0093-691X(01)00451-4
Ursula Süss, Kathelijne Wüthrich, Gerald Stranzinger, Chromosome configurations and time sequence of the first meiotic division in bovine oocytes matured in vitro. Biology of Reproduction. ,vol. 38, pp. 871- 880 ,(1988) , 10.1095/BIOLREPROD38.4.871
Kenneth C. Kleene, Evan Mulligan, Daniel Steiger, Kevin Donohue, Mary-Ann Mastrangelo, The mouse gene encoding the testis-specific isoform of Poly(A) binding protein (Pabp2) is an expressed retroposon: intimations that gene expression in spermatogenic cells facilitates the creation of new genes. Journal of Molecular Evolution. ,vol. 47, pp. 275- 281 ,(1998) , 10.1007/PL00006385