The structure and expression of maize genes encoding the major heat shock protein, hsp70

摘要: We have isolated and sequenced two maize genomic clones that are homologous to the Drosophila hsp70 gene. One of contains entire coding region 81 nucleotides 5' nontranslated sequence. The predicted amino acid sequence for this protein is 68% Drosophila. second clone only part 1.1 kb flanking This sequences consensus heat-shock-element Both genes thermally inducible each an intron in same position as heat-shock-cognate gene, hsc1, presence a distinguishing feature no other described date contain intron. constructed hybrid gene containing with intron, demonstrate transgenic petunia plant expressed from its own promoter.