Actin-cytoskeleton dynamics in non-monotonic cell spreading.
作者： Doris Heinrich , Simon Youssef , Britta Schroth-Diez , Ulrike Engel , Daniel Aydin
DOI: 10.4161/CAM.2.2.6190
关键词:
摘要: The spreading of motile cells on a substrate surface is accompanied by reorganization their actin network. We show that in the highly Dictyostelium non-monotonic, and thus differs from passage through regular series stages. Quantification gain loss contact area revealed fluctuating forces protrusion retraction dominate interaction with substrate. molecular basis these fluctuations elucidated dual-fluorescence labeling filamentous together proteins highlight specific activities system. Front-to-tail polarity established sorting out myosin-II regions where dense assemblies are accumulating. Myosin-IB identifies protruding front regions, Arp2/3 complex localizes to lamellipodia protruded regions. Coronin used as sensitive indicator disassembly visualize delicate balance polymerization ...
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