A Method for Rapid and Simultaneous Mapping of Genetic Loci and Introgression Sizes in Nematode Species

摘要: Caenorhabditis briggsae is emerging as an attractive model organism not only in studying comparative biology against C. elegans, but also developing novel experimentation avenues. In particular, recent identification of a new species, sp.9 with which it can mate and produce viable progeny provides opportunity for the genetics hybrid incompatibilities (HI) between two. Mapping specific HI locus demands repeated backcrossing to get hold genomic region underlying observed phenotype. To facilitate mapping loci sp.9, efficient method genetic map ideally consisting dominant markers are required systematic introgression fragments two species. We developed fast cost-effective high throughput resolution up 1 million bps briggsae. The takes advantage followed by PCR genotyping using primers. Importantly, results serve effective way estimating chromosomal position briggsae, size information fragment otherwise background. addition, helps generate line side-product that invaluable subsequent loci. will greatly construction pave isolation has so far been attempted nematode designed allele, be easily adapted any other type alleles species if sister pair feasible.