Tropism, susceptibility, infectivity, and cytokine releases of differentiated human tonsillar epithelial cells by different Influenza viruses

摘要: Human tonsil epithelium cells (HTEC) are a heterogeneous group of actively differentiating comprising stratified squamous epithelial and reticulated crypt with abundant keratin expression. We hypothesized that the tonsils primary site for influenza infection sustained viral replication. Primary HTEC were grown using an air-liquid culture infected apically different viruses (IVs) to measure growth kinetics. These cultures highly differentiated, subpopulations heterogenous surface rich both cilia microvilli; these contained more α2,6-linked sialic acids, those preferentially bound by human IVs, than α2,3-linked avian like acids. The interrupted patches reticular in permissive A B virus Following infection, subset cells, mostly ciliated underwent apoptosis while others remained intact despite being positive IV nucleoprotein. H3N2 antigen colocalized non-ciliated H1N1 was associated cells. Exposure HTECs IVs triggers early proinflammatory response fluctuates between viruses. induces persists, whereas pH1N1 primarily late HTECs. Our results implicated as differentiated system provides valuable vitro model studying cellular tropism, infectivity, cytokine responses pathogenesis IVs.