Glutathione and Homoglutathione Synthesis in Legume Root Nodules

摘要: High-performance liquid chromatography (HPLC) with fluorescence detection was used to study thiol metabolism in legume nodules. Glutathione (GSH) the major non-protein all indeterminate nodules examined, as well determinate of cowpea (Vigna unguiculata), whereas homoglutathione (hGSH) predominated soybean (Glycine max), bean (Phaseolus vulgaris), and mungbean radiata) All had greater concentrations than leaves roots same plants because active synthesis nodule tissue. The correlation between tripeptides activities glutathione synthetase (GSHS) (hGSHS) eight legumes, contrasting contents alfalfa (Medicago sativa) (98% hGSH, 100% hGSHS) (72% GSH, 80% GSHS) indicated that distribution GSH hGSH is determined by specific synthetases. Thiol decreased both natural induced senescence, were also reduced senescent zone Thiols GSHS especially abundant meristematic infected zones pea (Pisum sativum) γ-glutamylcysteinyl more nodules, but hGSHS predominant cortex. Isolation full-length cDNA sequences coding for from revealed they are highly homologous those other higher plants.