Dynactin increases the processivity of the cytoplasmic dynein motor

摘要: Cytoplasmic dynein supports long-range intracellular movements of cargo in vivo but does not appear to be a processive motor protein by itself. We show here that the activator, dynactin, binds microtubules and increases average length cytoplasmic-dynein-driven without affecting velocity or microtubule-stimulated ATPase kinetics cytoplasmic dynein. Enhancement microtubule binding motility dynactin are both inhibited an antibody dynactin's microtubule-binding domain. These results indicate acts as processivity factor for cytoplasmic-dynein-based provide first evidence cytoskeletal can affected extrinsic factors.