Sexual crossing of thermophilic fungus Myceliophthora heterothallica improved enzymatic degradation of sugar beet pulp

摘要: Enzymatic degradation of plant biomass requires a complex mixture many different enzymes. Like most fungi, thermophilic Myceliophthora species therefore have large set enzymes targeting linkages in polysaccharides. The majority these not been functionally characterized, and their role is unknown. biotechnological challenge to select the right efficiently degrade particular biomass. This study describes strategy using sexual crossing screening with fungus heterothallica identify specific associated improved sugar beet pulp saccharification. Two genetically diverse M. strains CBS 203.75 663.74 were used generate progenies growth on pulp. One progeny, named SBP.F1.2.11, had genetic pattern from parental saccharification activity after 3 % SBP was explained by altered activities major (hemi-)cellulases. Exo-proteome analysis progeny 7-day showed that only 17 133 secreted CAZy more abundant SBP.F1.2.11. Particularly one enzyme belonging carbohydrate esterase family 5 (CE5) CE5-CBM1 enzyme, as Axe1, phylogenetically related acetyl xylan esterases. Biochemical characterization Axe1 confirmed de-acetylation optimal at 75–85 °C pH 5.5–6.0. Supplementing release xylose glucose identified beneficial for selecting this substrate. Saccharification supplementing mixtures previously uncharacterized esterase. shows selection are successful improve composition efficient degradation.