Insights into the osteoblast precursor differentiation towards mature osteoblasts induced by continuous BMP-2 signaling

摘要: Mature osteoblasts are the cells responsible for bone formation and derived from precursor osteoblasts. However, mechanisms that control this differentiation poorly understood. In fact, unlike majority of organs in body, which composed "soft" tissue can easily be isolated studied, "hard" mineralized has made it difficult to study function cells. Here, we established an vitro model mimics under physiological conditions. We obtained mature characterized them on basis following parameters: strong expression osteoblastic markers, such as Runx2 Col-I; achievement specific dimensions (the cell volume increases 26-fold compared osteoblast precursors); production abundant extracellular matrix also called osteoid. demonstrated precursors into requires continuous activation Bone Morphogenetic Protein (BMP) receptors, with immobilization a BMP-2mimetic peptide synthetic mimicking vivo microenvironment. Importantly, organization F-actin network acetylated microtubules were modified during process. showed perturbation cytoskeleton abolished addition, gene is required differentiation. These findings demonstrate retro-regulation cytoplasmic genic components due induction BMP-2 provide more detailed insights correct signaling BMPs tissue.